烟草种间杂交亲和性

以野生烟草Nicotiana alata、N.rustica、N.repanda、N.stocktonnii与栽培烟草K326、红花大金元、Yun87、Yun97为材料，进行种间正反杂交，研究种间杂交亲和性。田间观察杂交后的坐果情况并统计坐果率，采用显微荧光染色观察授粉后花粉管在雌蕊上的生长情况，并结合杂交后代萌发检测的方法。结果表明：N.rustica、N.repanda、N.stocktonnii与栽培烟草杂交不亲和。N.rustica花粉能够穿过K326花柱，N.repanda和N.stocktonnii花粉在K326柱头上很少萌发生长。N.alata花粉可以穿过K326的花柱，并得到果实，但是萌发实验显示其种子无活力。N.alata作为母本与栽培烟草杂交不亲和。

Cross compatibility of Interspecific Cross in Tobacco

Four wild species of tobacco (Nicotiana alata, N.rustica, N.repanda and N.stocktonnii) and four flue cured tobacco (K326, Hongda, Yun87, Yun97), were used as experimental materials in this research. The interspecific reciprocal crosses were carried out in order to study the compatibility. The seed setting percentage of cross was counted in field, and then test seed germination. Pollen tube behaviors were observed via fluorescence microscope after pollination. The results indicated that N.rustica, N.repanda and N.stocktonnii were incompatibility with the flue cured tobacco. The pollen of N.rustica passed through the pistil of K326, but the pollens of N.repanda and N.stocktonnii were rarely growth on the pistil of K326. In spite of the pollen of N.alata passed through the pistil of K326, the hybrid tobacco seed had no vigor by the seed germination test. The female of N.alata was incompatibility with the flue cured tobacco.