提高成年云南山楂树试管培养苗生根率的方法

用成年云南山楂(Crataegus scabrifolia(Franch)Rehd.树的无菌增殖芽苗作为生根试验材料。结果表明：接种在含生长素(IBA、NAA)0.01-1.0mg/1的MS/2培养基中，芽苗的平均生根率为50.4%；在高浓度(150-250mg/1)生长素溶液中浸泡芽苗基部30分钟，然后接种到无生长素的MS/2培养基中，其生根率为60.1%；用高浓度生长素液蘸芽苗基部的生根率为83.5%；芽苗在含IBA1-5mg/1的培养基中培养2-4天，然后转入MS/2培养基中诱导生根，生根率可达92%以上，根伸长正常。黑暗条件明显抑制生根，每日16小时至24小时光照对芽苗生根有益。

STUDIES ON THE GEOGRAPHICAL PROVENANCE OF PINUS YUNNANENSIS I.SEEDLING TEST

A scedling test including tbe mcasure and analysis of variance of the height and diameter at ground level of 21-months old seedlings of 27 different provenances of Pinus yunnanensis Fr. was carried out. The result showed that the variations of the height and diameter of different provenances of Pinus yun- nonensis planted in the same cxperimental plot were very remarkable. We have primarily selected 10 provenances of Pinus yunnanensis of which the height increment are faster than those of control and which can be used in forest cxperi- ment. In addition to a primary study of the geographical variations of morpho- logical characteristics and their relationship also described.