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Scanning electron microscopic examination of a pellet formulation of Bacillus megaterium and B. pumilus,antagonists of Rhizoctonia solani,and survival during storage
Authors:Kanjanamaneesathian  Mana  Pengnoo  Ashara  Jantharangsri  Apinya  Niratana  Ladda  Kusonwiriyawong  Chirasak
Institution:(1) Department of Pest Management, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand;(2) Department of Earth Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand;(3) Scientific Equipment Centre, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand;(4) Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand;(5) Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand
Abstract:Bacterial formulations, produced using both Bacillus megaterium and B. pumilus individually with pharmaceutical technology, were formulated using a wet granular method. Viability testing in the laboratory revealed that bacterial populations rapidly declined during storage at room temperature (26–30 °C) for 6 months. The scanning electron microscope (SEM) was used to observe bacterial formulations. Both endospores and vegetative cells of B. megaterium and B. pumilus were detected on the formulation surfaces. This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:Bacillus megaterium                      B  pumilus            biological control  pellet formulation            Rhizoctonia solani            storage  survival
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