Genome size and sequence composition of moso bamboo: A comparative study |
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Authors: | GUI YiJie WANG Sheng QUAN LiYan ZHOU ChangPing LONG ShiBao ZHENG HuaJun JIN Liang ZHANG XianYin MA NaiXun & FAN LongJiang
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Institution: | (1) Institute of Crop Science/Institute of Bioinformatics, Zhejiang University, Hangzhou, 310029, China;(2) Zhejiang Anji Bamboo Exposition Garden, Anji, 313300, China;(3) Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China;(4) The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, 311400, China |
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Abstract: | Moso bamboo (Phyllostachys pubescens) is one of the world’s most important bamboo species. It has the largest area of all planted bamboo—over two-thirds of the
total bamboo forest area—and the highest economic value in China. Moso bamboo is a tetraploid (4x=48) and a special member of the grasses family. Although several genomes have been sequenced or are being sequenced in the
grasses family, we know little about the genome of the bambusoids (bamboos). In this study, the moso bamboo genome size was
estimated to be about 2034 Mb by flow cytometry (FCM), using maize (cv. B73) and rice (cv. Nipponbare) as internal references.
The rice genome has been sequenced and the maize genome is being sequenced. We found that the size of the moso bamboo genome
was similar to that of maize but significantly larger than that of rice. To determine whether the bamboo genome had a high
proportion of repeat elements, similar to that of the maize genome, approximately 1000 genome survey sequences (GSS) were
generated. Sequence analysis showed that the proportion of repeat elements was 23.3% for the bamboo genome, which is significantly
lower than that of the maize genome (65.7%). The bamboo repeat elements were mainly Gypsy/DIRS1 and Ty1/Copia LTR retrotransposons
(14.7%), with a few DNA transposons. However, more genomic sequences are needed to confirm the above results due to several
factors, such as the limitation of our GSS data. This study is the first to investigate sequence composition of the bamboo
genome. Our results are valuable for future genome research of moso and other bamboos.
Supported by the Program for New Century Excellent Talents in University of China |
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Keywords: | flow cytometry (FCM) genome size GSS Phyllostachys pubescens repeat elements |
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