Molecular cloning and characterization of Toll-like receptor 14 in Japanese flounder,Paralichthys olivaceus |
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| Authors: | Seong Don Hwang Hidehiro Kondo Ikuo Hirono Takashi Aoki |
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| Affiliation: | 1. Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China;2. State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, PR China;3. Department of Internal Medicine, College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA;1. The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849, USA;2. Central Institute of Fisheries Education (CIFE), Versova, Andheri (W), Mumbai 400061, India;1. Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK;2. Marine Scotland, 375 Victoria Road, Aberdeen AB11 9DB, UK;3. Zoetis International Service, 23-25 Avenue du Dr. Lannelongue 75668 Paris Cedex 14, France;1. Guangzhou Airport Extry-Exit Inspection and Quarantine Bureau, Guangzhou 510470, Guangdong Province, PR China;2. College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China;3. College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China;1. Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK;2. Zoetis International Services, Animal Health Division, 23-25 Avenue du Dr. Lannelongue, 75668 Paris Cedex 14, France;1. Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, People''s Republic of China;2. Aquatic Technology Promoting Station of Meijiang District, Meizhou, 514000, People''s Republic of China |
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| Abstract: | Toll-like receptors (TLRs) are essential for activation of the innate immune system in response to invading pathogens. TLR14, which is unique to fish, has been identified in several fish species, but its function is unclear. In this study, Japanese flounder (Paralichthys olivaceus) TLR14 gene (JfTLR14) was cloned and its expression profiles were analyzed after infection with viral hemorrhagic septicemia virus, gram-positive Streptococcus iniae and gram-negative Edwardsiella tarda. The coding region of JfTLR14 cDNA was 2,607 bp, encoding 878 amino acid residues. JfTLR14 was highly expressed in head kidney of healthy flounder. In response to infection with VHSV and S. iniae, the JfTLR14 gene was up-regulated at only 1 day post-infection (dpi). However, E. tarda infection increased JfTLR14 gene expression from 1 to 6 dpi. These results imply that JfTLR14 participates more in the immune response against E. tarda infection than in the immune responses to other pathogen infections. |
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