The Effect of Leaf Temperature and Photorespiratory Conditions on Export of Sugars during Steady-State Photosynthesis in Salvia splendens

Export and photosynthesis in leaves of Salvia splendens were measured concurrently during steady-state 14CO2 labeling conditions. Under ambient CO2 and O2 conditions, photosynthesis and export rates were similar at 15 and 25deg]C, but both declined as leaf temperature was raised from 25 to 40deg]C. Suppressing photorespiration between 15 and 40deg]C by manipulating CO2 and O2 levels resulted in higher rates of leaf photosynthesis, total sugar synthesis, and export. There was a linear relationship between the rate of photosynthesis and the rate of export between 15 and 35deg]C. At these temperatures, 60 to 80% of the carbon fixed was readily exported with sucrose, raffinose, and stachyose, which together constituted over 90% of phloem mobile assimilates. Above 35deg]C, however, export during photosynthesis was inhibited both in photorespiratory conditions, which inhibited photosynthesis, and in nonphotorespiratory conditions, which did not inhibit photosynthesis. Sucrose and raffinose but not stachyose accumulated in the leaf at 40deg]C. When leaves were preincubated at 40deg]C and then cooled to 35deg]C, export recovered more slowly than photosynthesis. These data are consistent with the view that impairment of export processes, rather than photosynthetic processes associated with light trapping, carbon reduction, and sucrose synthesis, accounted for the marked reduction in export between 35 and 40deg]C. Taken together, the data indicated that temperature changes between 15 and 40deg]C had two effects on photosynthesis and concurrent export. At all temperatures, suppressing photorespiration increased both photosynthesis and export, but above 35deg]C, export processes were more directly inhibited independent of changes in photorespiration and photosynthesis.