Efficient heterodimerization of recombinant bi- and trispecific antibodies |
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Authors: | R Schoonjans A Willems J Grooten N Mertens |
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Institution: | (1) Molecular Immunology Unit, VIB1 – Department of Molecular Biology, University of Gent, K.L.Ledeganckstraat 35, B-9000 Gent, Belgium |
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Abstract: | Bispecific antibodies (BsAb) are promising therapeutic tools in tomorrow's medicine. Expression systems favoring efficient heterodimerization of intermediate-sized bispecific antibodies will significantly improve existing production methods. By C-terminal fusion of scFv molecules to the Fd- and the L-chains efficient heterodimerization in mammalian cells was obtained and a novel intermediate sized, disulfide stabilized BsAb could be efficiently produced. This type of antibody derivative easily allows for the production of trispecific antibodies, BsAb with bivalent binding for one antigen, or immunoconjugates. |
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Keywords: | bispecific antibody cancer iummunotherapy heterodimerization immunoconjugate recombinant gene expression |
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