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Sequence of the cellular T-DNA in the untransformed genome of Nicotiana glauca that is homologous to ORFs 13 and 14 of the Ri plasmid and analysis of its expression in genetic tumors of N. glauca x N. langsdorffii |
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| Authors: | Seishirō Aoki Akiyoshi Kawaoka Masami Sekine Takanari Ichikawa Tomomichi Fujita Atsuhiko Shinmyō Kunihiko Syōno |
| Institution: | 1. Departments of Pure and Applied Sciences and Biology, University of Tokyo, Meguro-ku, 153, Tokyo, Japan 2. Research Laboratory of Bioscience, Nippon Paper Industries Co. Ltd, 740, Iwakuni, Japan 3. Department of Biotechnology, Faculty of Engineering, Osaka University, Suita-shi, 565, Osaka, Japan 4. Friedrich Miescher-Institut, CH-4002, Basel, Switzerland |
| Abstract: | A region homologous to the TL-DNA of Agrobacterium rhizogenes was previously detected in the genome of untransformed Nicotiana glauca and designated cellular T-DNA (cT-DNA). Subsequently, part of this region was sequenced and two genes, which corresponded to rolB and rolC and were named NgrolB and NgrolC, were found. We have now sequenced a region of the cT-DNA other than the region that includes NgrolB and C and we have found two other open reading frames (ORFs), NgORF13 and NgORF14. These ORFs correspond to ORFs 13 and 14 of the TL-DNA of A. rhizogenes and exhibit a high degree of homology to these ORFs, without having a nonsense codon. We have not found any sequence homologous to rolD (ORF15). The two genes, NgORF13 and 14, as well as the NgrolB and C genes, are expressed in genetic tumors of hybrids between N. glauca and N. langsdorffii but not in leaf tissues of the hybrid. |
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