Effect of iron and EDTA on ethyl acetate accumulation in Candida utilis |
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| Authors: | David W Armstrong Hiroshi Yamazaki |
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| Institution: | (1) Division of Biological Sciences, National Research Council of Canada, K1A OR6 Ottawa, Canada;(2) Department of Biology, Carleton University, K1S 5B6 Ottawa, Canada |
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| Abstract: | Summary Production of economically-recoverable products from dilute sugar or ethanol is of practical importance. Conversion of glucose to ethyl acetate by Candida utilis was inhibited by FeCl3 supplementation as low as 10 uM. EDTA added at the onset of growth on glucose relieved such an inhibition and also caused faster and greater amounts of accumulation of the ester. Addition of EDTA during conversion of ethanol to ethyl acetate showed little effect. EDTA may affect cell permeability and/or oxidative metabolism. For continual ethyl acetate production iron limitation must be maintained during as well as before ethanol utilization.EDTA = Ethylenediaminetetraacetic acid. |
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