Characterization of pbpA and pbp2 Encoding Penicillin-binding Proteins Located on the Downstream of Clavulanic Acid Gene Cluster in Streptomyces clavuligerus |
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| Authors: | Kenji Ishida Trinh Viet Hung Kwangkyoung Liou Hei Chan Lee Chang-Hun Shin Jae Kyung Sohng |
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| Institution: | (1) Institute of Biomolecule Reconstruction, Sun Moon University, 100, Kalsanri, Tangjeonmyun, 336-708 Asansi, Chungnam, Korea;(2) CDK BIO Research Institute 454, Asansi, Kyunggi, Korea |
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| Abstract: | Two genes, pbpA (orf18) and pbp2 (orf19) located on the downstream of clavulanic acid (CA) gene cluster of Streptomyces clavuligerus were cloned into pET-28a(+), and confirmed to encode a family of high molecular-weight penicillin-binding proteins (PBPs).
Both genes were amplified from genomic DNA by PCR and expressed in E. coli BL21 (DE3). Hydropathy plots of the proteins revealed a single stretch of hydrophobic amino acids indicating them to be transmembrane
proteins. Pbp2 had lower affinity to penicillin G compared to PbpA, and was essential to the cell growth in contrast to PbpA.
Revisions requested 3 November 2005; Revisions received 13 December 2005 |
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| Keywords: | Actinomycetes clavulanic acid penicillin-binding protein Streptomyces clavuligerus |
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