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Exoamylase activity in vacuoles isolated from pea and wheat leaf protoplasts
Authors:Ziegler P  Beck E
Affiliation:Lehrstuhl Pflanzenphysiologie, Universität Bayreuth, Universitätsstraβe 30, D-8580 Bayreuth, Federal Republic of Germany
Abstract:Vacuoles isolated from pea (Pisum sativum), and wheat (Triticum aestivum) leaf protoplasts contained considerable activities of electrophoretically highly mobile exoamylases. Vacuoles from spinach (Spinacia oleracea) leaf and photoautotrophic Chenopodium rubrum suspension culture cell protoplasts were devoid of amylolytic activity. Endoamylase activity was in all cases associated primarily with the chloroplast.
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