| 山羊小腔卵泡DNA含量的荧光分析 |
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| 引用本文： | 李键,康靖全,周乐,王建辰.山羊小腔卵泡DNA含量的荧光分析[J].生物化学与生物物理进展,1999,26(2):182-184. |
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| 作者姓名： | 李键 康靖全 周乐 王建辰 |
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| 作者单位： | 1. 四川省畜牧科学研究院,成都,610066
2. 西北农业大学动物医学系,杨陵,712100 |
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| 基金项目： | 国家自然科学基金资助项目(39470519). |
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| 摘 要： | 用显微手术法剥离山羊3 mm以下完整小腔卵泡,用胶原酶和Triton X-100进行处理,选用荧光试剂4,6-二脒基-2-苯吲哚(4,6-diamidino-2-phenylindol-2HCl,DAPI),对其DNA含量进行分析.结果表明,山羊3 mm以下卵泡的DNA含量与卵泡直径相关性极显著(r=0.9824＞0.7800=r0.01,n=12).该法可测定5 ng的DNA.提示该法在卵泡生长发育的研究中具有重要应用价值.
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| 关 键 词： | 荧光分析 DNA 卵泡 山羊 |
| 收稿时间： | 1997/12/4 0:00:00 |
| 修稿时间： | : 1997-12- |
Fluorometric Assay for DNA in Goat Small Antral Follicles |
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| LI Jian,KANG Jin-Quan,ZHOU Le and WANG Jian-Chen.Fluorometric Assay for DNA in Goat Small Antral Follicles[J].Progress In Biochemistry and Biophysics,1999,26(2):182-184. |
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| Authors: | LI Jian KANG Jin-Quan ZHOU Le and WANG Jian-Chen |
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| Institution: | Department of Veterinary Sciences, North West Agricultural University, Yangling,Shaanxi 712100,China;Department of Veterinary Sciences, North West Agricultural University, Yangling,Shaanxi 712100,China;Department of Veterinary Sciences, North West Agricultural University, Yangling,Shaanxi 712100,China;Department of Veterinary Sciences, North West Agricultural University, Yangling,Shaanxi 712100,China |
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| Abstract: | Intact goat small antral follicles were dissected from ovaries by a micro surgical method. The follicular DNA content was estimated by a fluorometric method using the fluorochrome , 4,6 diamidino 2 phenylindol 2HCl(DAPI).Significant positive correlations were found between the follicular diameters and follicular DNA contents in follicles smaller than 3 mm ( r =0 9824>0 7800= r 0 001 , n =12). It suggested that this method of DNA assay would have great application value in study on follicular gowth and development. |
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| Keywords: | fluorometry DNA follicle goat |
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