皖南地区丙型肝炎病毒治疗前后HVR1区碱基变异数量的研究

目的研究皖南地区丙型肝炎病毒治疗前后高变区1(HVR1)碱基变异数量与病情变化和治疗效果之间是否具有相关性。方法应用速率法和荧光定量PCR法,检测皖南地区141例慢性丙型肝炎患者血清ALT水平和HCV RNA的载量。应用RT-PCR法进行HCV基因型和治疗前后的HVR1区域序列的检测。结果 141例患者的血清ALT与HCV RNA两指标间采用Spearman等级相关分析显示,rs=0.213,P=0.011,差异具有统计学意义,存在正相关。通过HCV基因1型、2型和未分型3组的HVR1碱基变异数量的单因素方差分析,显示组与组之间差异具有统计学意义。结论慢性丙型肝炎患者治疗前后的HVR1序列碱基变异数量的分析,对治疗效果、病情变化及预后发展等方面的评估具有重要的指导意义。

Study on the number of base variation of HVR1 region of Hepatitis C in the south of Anhui province after treatment

Objective To study the correlation among the number of base variation of HVR1 region of Hepatitis C Virus,disease activity and therapeutic effect in the south of Anhui province after treatment.Method Rate method and real-time PCR were used to detect ALT and the HCV-RNA level in 141 serum samples of chronic Hepatitis C in the south of Anhui province.RT-PCR was used to detect the HCV genotype and the number of base variation of HVR1 region after treatment.Result The level of ALT were positive correlated with the level of HCV-RNA（rs=0.213,P=0.011）.The number of base variation of HVR1 region was statistically significant among the three groups aboutⅠ,Ⅱand non-branched genotype of Hepatitis C.Conclusion The study on the number of base variation of HVR1 region after treatment plays a significant role in evaluating the therapeutic effect,disease activity and prognosis of Hepatitis C.