Molecular Cloning and Characterization of Rubber Biosynthetic Genes from <Emphasis Type="Italic">Taraxacum koksaghyz</Emphasis> |
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Authors: | Thomas Schmidt Andrea Hillebrand David Wurbs Daniela Wahler Malte Lenders Christian Schulze Gronover Dirk Prüfer |
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Institution: | 1.Institut für Biochemie und Biotechnologie der Pflanzen,Westf?lische Wilhelms-Universit?t Münster,Münster,Germany;2.Fraunhofer Institut für Molekularbiologie und Angewandte ?kologie,Aachen,Germany |
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Abstract: | Rubber biosynthesis requires the action of specific enzymes known as cis-prenyltransferases (CPTs). These enzymes are responsible for the sequential addition of isopentenyl pyrophosphate units to
the growing polyisoprene chain, a biochemical reaction thought to be stimulated by the presence of small rubber particle proteins
(SRPPs). We have cloned, characterized, and analyzed the expression of three CPT genes (TkCPT1–3) and five SRPP genes (TkSRPP1–5) from the rubber-producing plant Taraxacum koksaghyz. The deduced TkCPT amino acid sequences showed significant levels of sequence identity with Hevea brasiliensis CPTs. We also found no obvious differences between SRPPs from T. koksaghyz, another rubber producer, and a non-rubber plant. The roles of the individual TkCPTs and TkSRRPs in rubber biosynthesis are
discussed. |
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