Changes of Relative Weight and Cell Cycle,and Lesions of Bursa of Fabricius Induced by Dietary Excess Vanadium in Broilers |
| |
Authors: | Wei Cui Hengmin Cui Xi Peng Jing Fang Zhicai Zuo Xiaodong Liu Bangyuan Wu |
| |
Institution: | (1) Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China, 625014; |
| |
Abstract: | The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on immune function by determining
the morphological changes and cell cycle of bursa of Fabricius, and the serum Ig contents. A total of 420 one-day-old avian
broilers were divided into six groups and fed on a corn–soybean basal diet as control diet, or the same diet amended to contain
5, 15, 30, 45, and 60 ppm vanadium supplied as ammonium metavanadate. When compared with that of control group, the relative
weight of bursa was significantly increased in the 15 ppm group from 14 to 35 days of age and increased in the 5 ppm group
at 42 days of age, and significantly decreased in the 60 ppm group from 14 to 42 days of age and decreased in 30 and 45 ppm
groups from 35 to 42 days of age. Pathological lesions progressed as the dietary vanadium increased. The gross lesions of
bursa showed obvious atrophy with decreased volume and pale color in 45 and 60 ppm groups. Histopathologically, widened cortex
and increased number of lymphocytes appeared in 5 and 15 ppm groups, and reduced lymphocytes and connective tissue hyperplasia
appeared in 45 and 60 ppm groups. The bursal cells in static phase (G0/G1) were decreased, and those in the mitotic phase (G2 + M) and the proliferating index (PI) were increased in 5 and 15 ppm groups. However, bursal cells in the G0/G1 phase were increased, and those in G2 + M phase, synthesis phase (S) and the PI were decreased in 45 and 60 ppm groups. Also, the serum IgG and IgA contents were
increased in 5 and 15 ppm groups, and the serum IgG, IgA, and IgM contents were decreased in 45 and 60 ppm groups. These results
suggested that dietary excess vanadium (45 and 60 ppm) could inhibit growth of bursa of Fabricius and impair humoral immunity
in chicken. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|