Kinetin inhibits protein oxidation and glycoxidation in vitro |
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Authors: | Verbeke P Siboska G E Clark B F Rattan S I |
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Institution: | Department of Molecular and Structural Biology, Danish Centre for Molecular Gerontology, Laboratory of Cellular Ageing, Gustav Wieds Vej 10-C, DK-8000, Denmark. |
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Abstract: | We tested the ability of N(6)-furfuryladenine (kinetin) to protect against oxidative and glycoxidative protein damage generated in vitro by sugars and by an iron/ascorbate system. At 50 microM, kinetin was more efficient (82% inhibition) than adenine (49% inhibition) to inhibit the bovine serum albumin (BSA)-pentosidine formation in slow and fast glycation/glycoxidation models. Kinetin also inhibited the formation of BSA-carbonyls after oxidation significantly more than adenine did. However both compounds inhibited the advanced glycation end product (AGE) formation to the same extent (59-68% inhibition). At 200 microM, kinetin but not adenine, limited the aggregation of BSA during glycation. These data suggest that kinetin is a strong inhibitor of oxidative and glycoxidative protein-damage generated in vitro. |
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