Microarray analysis reveals a potential role of LncRNAs expression in cardiac cell proliferation |
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Authors: | Jue Wang Zhimin Geng Jiakan Weng Longjie Shen Ming Li Xueli Cai Chengchao Sun Maoping Chu |
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Institution: | 1.Department of Cardiac Surgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,People’s Republic of China;2.Children’s Heart Center, the Second Affiliated Hospital & Yuying Children’s Hospital, Institute of Cardiovascular Development and Translational Medicine,Wenzhou Medical University,Wenzhou,People’s Republic of China;3.Tianjin Childrens’ Hospital,Tianjin,People’s Republic of China;4.Department of Transplantation,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,People’s Republic of China;5.Cardiac Regeneration Research Institute, Wenzhou Medical University,Wenzhou,People’s Republic of China;6.Department of Cardiology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,People’s Republic of China |
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Abstract: | BackgroundLong non-coding RNAs (LncRNAs) have been identified to play important roles in epigenetic processes that underpin organogenesis. However, the role of LncRNAs in the regulation of transition from fetal to adult life of human heart has not been evaluated.MethodsImmunofiuorescent staining was used to determine the extent of cardiac cell proliferation. Human LncRNA microarrays were applied to define gene expression signatures of the fetal (13–17 weeks of gestation, n?=?4) and adult hearts (30–40 years old, n?=?4). Pathway analysis was performed to predict the function of differentially expressed mRNAs (DEM). DEM related to cell proliferation were selected to construct a lncRNA-mRNA co-expression network. Eight lncRNAs were confirmed by quantificational real-time polymerase chain reaction (n?=?6).ResultsCardiac cell proliferation was significant in the fetal heart. Two thousand six hundred six lncRNAs and 3079 mRNAs were found to be differentially expressed. Cell cycle was the most enriched pathway in down-regulated genes in the adult heart. Eight lncRNAs (RP11-119 F7.5, AX747860, HBBP1, LINC00304, TPTE2P6, AC034193.5, XLOC_006934 and AL833346) were predicted to play a central role in cardiac cell proliferation.ConclusionsWe discovered a profile of lncRNAs differentially expressed between the human fetal and adult heart. Several meaningful lncRNAs involved in cardiac cell proliferation were disclosed. |
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