A simple and rapid preparation of alditol acetates for monosaccharide analysis |
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| Authors: | Anthony B Blakeney Philip J Harris Robert J Henry Bruce A Stone |
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| Institution: | Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083 Australia |
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| Abstract: | A simple and rapid method is described for the preparation of alditol acetates from monosaccharides. It can be performed in a single tube without transfers or evaporations. Monosaccharides are reduced with sodium borohydride in dimethyl sulphoxide and the resulting alditols acetylated using 1-methylimidazole as the catalyst. Removal of borate is unnecessary and acetylation is complete in 10 min at room temperature. Monosaccharides are quantitatively reduced and acetylated by this procedure. The alditol acetates are completely separated by glass-capillary, gas-liquid chromatography on Silar 10C. The method has been applied to the analysis of monosaccharides in acid hydrolysates of a plant cell-wall. |
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| Keywords: | To whom correspondence should be addressed |
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