增强恶性疟原虫DNA疫苗免疫反应的研究

构建了含有恶性疟原虫抗原基因 ( AWTE)及白介素 2基因的重组质粒 p CMV- AWTE以及p CMV- IL2、p CMV- IL2 - AWTE、p RSV- AWTE。将纯化的质粒混合后免疫小鼠 ,3次免疫后比较其诱导机体产生的免疫应答的水平 ,发现 IL- 2可以明显地提高机体的细胞免疫 ,而对体液免疫的影响甚微。麻醉剂、蔗糖、免疫剂量等因素也可以不同程度地提高机体的免疫应答水平 ,RSV启动子与 CMV启动子对免疫应答水平无明显的影响

Factors improving the efficacy of DNA vaccine

Cholera toxin B subunit is a good carrier protein and an effective adjuvant which can boost both cellular and humoral immunity. DNA fragments encoding B cell, Th cell and CTL epitopes of P.falciparum CS, MSA 1, MSA 2 and RESA antigens were cloned down 2 stream of cholera toxin B subunit gene in the same reading frame. Another modification using IL2 as adjuvant was also made. We studied factors improving the efficacy of DNA vaccine including bupivacaine, sucrose,administration dose etal through comparing antibody leval and CTL activity after plasmid immunization.