| 结直肠癌CD133的表达与细胞周期蛋白水平及血清癌胚抗原含量的关系 |
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| 引用本文： | 于冬冬,张永红,邹游,陶德定,胡俊波,龚建平.结直肠癌CD133的表达与细胞周期蛋白水平及血清癌胚抗原含量的关系[J].中国组织化学与细胞化学杂志,2010,19(6):553-557. |
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| 作者姓名： | 于冬冬 张永红 邹游 陶德定 胡俊波 龚建平 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院分子医学中心,武汉,430030 |
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| 摘 要： | 目的探讨结直肠癌肿瘤干细胞标志物CD133分子的表达与细胞周期蛋白表达有无关系,分析肿瘤CD133分子的表达变化是否影响血清肿瘤标志物癌胚抗原的水平。方法应用流式细胞术检测41例结直肠癌肿瘤组织的CD133,cyclin D1,cyclin E,cyclin A和cyclin B1的表达水平,血清癌胚抗原的含量常规由检验科用放免法检测,数据采集自检验报告单。结果 41例结直肠癌肿瘤组织CD133分子的表达率是0.3%-47.07%,cyclin D1为2.94%-36.93%,cyclin E为0.49%-9.28%,cyclin A为0.42%-22.33%,cyclin B1为1.24%-22.96%。相关分析表明,CD133的表达水平与cyclinD1相关r=0.405 P=0.047,与其它几种细胞周期蛋白的表达水平无关,均为P0.05。CD133的表达水平和血清癌胚抗原的表达呈正相关r=0.601P=0.009。结论结直肠癌CD133分子的表达与细胞周期蛋白cyclin D1的表达正相关,两者一定程度上共同反映了癌细胞的增殖状态。CD133高表达的结直肠癌,常伴有患者血清癌胚抗原的高表达,两者密切相关。
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| 关 键 词： | CD133 肿瘤干细胞 细胞周期蛋白 流式细胞术 癌胚抗原 |
RELATIONSHIP OF CD133 EXPRESSION WITH CYCLINS AND SERUM CARCINO-EMBRYONIC ANTIGEN IN COLORECTAL CANCER |
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| Yu Dongdong,Zhang Yonghong,Zou You,Tao Deding,Hu Junbo,Gong Jianping.RELATIONSHIP OF CD133 EXPRESSION WITH CYCLINS AND SERUM CARCINO-EMBRYONIC ANTIGEN IN COLORECTAL CANCER[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(6):553-557. |
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| Authors: | Yu Dongdong Zhang Yonghong Zou You Tao Deding Hu Junbo Gong Jianping |
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| Institution: | Yu Dongdong,Zhang Yonghong,Zou You,Tao Deding,Hu Junbo,Gong Jianping(Molecular Medical Centre,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China) |
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| Abstract: | Objective To investigate the association of CD133 expression with cyclins,and to study whether serum carcino embryonic antigen level can be influenced by CD133 expression.Methods The expressions of CD133,cyclinD1,cyclinE,cyclinA and cyclinB1 were detected by flow cytometry in 41 colorectal cancer tissues.Serum carcinoembryonic antigen level was detected by radioimmunoassay.Results In the 41 colorectal cancer tissues analyzed,CD133 expression varied from 0.3% to 47.07%.cyclin D1 2.94%-36.93%,cyclin E 0.49%-9... |
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| Keywords: | Antigen CD133 Cancer stem cell Cyclin Flow cytometry Carcino embryonic Antigen |
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