In vivo and in vitro association of 14-3-3 epsilon isoform with calmodulin: implication for signal transduction and cell proliferation |
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Authors: | Luk S C Ngai S M Tsui S K Fung K P Lee C Y Waye M M |
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Affiliation: | Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T. |
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Abstract: | Using a yeast two-hybrid screen, human 14-3-3 epsilon protein was found to interact with human calmodulin. In vitro binding assay between human 14-3-3 epsilon protein/peptide and calmodulin was demonstrated by native gel electrophoresis, and the interaction was shown to be calcium dependent. Our results, along with the association of the 14-3-3 epsilon protein with other signaling proteins, suggest that the 14-3-3 protein could provide a link between signal transduction and cell proliferation. |
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