Changes in protein profile and amino acids in <Emphasis Type="Italic">Cladophora vagabunda</Emphasis> (Chlorophyceae) in response to salinity stress |
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Authors: | G Rani |
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Institution: | (1) Reader, S.D.N.B. Vaishnav College for Women, Chromepet, Chennai, 600 044, India |
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Abstract: | The aim of the present study was to detect organic substances functioning as osmoticants that are used by the intertidal alga,
Cladophora vagabunda (L.) Hoek (Chlorophyceae), to adapt to a wide range of salinity. The major constituents of the amino acid pool were aspartate,
glutamate, glycine, valine, lysine, histidine, arginine, and proline. There were concomitant increases in the acidic amino
acids: aspartate and the glutamate and the basic amino acids: lysine, histidine and arginine in response to salinity stress.
The appearance of proline at hypersalinity alone showed that it acts as an osmoticant. As salinity increased, there was a
progressive shift in the electrophoretic pattern of protein bands. New peptide bands appeared under hyposalinity (10‰) and
hypersalinity (65‰) stress conditions in addition to the usual bands which appeared in the control (35‰). Glycine betaine,
which has been considered a novel organic osmolyte in a number of organisms, has also been observed in C. vagabunda in response to salinity stress. The synthesis of the compatible solute glycine betaine and the amino acid proline with increasing
salinity illustrates the contention that marine algae establish an osmotic equilibrium primarily by the synthesis of organic
compounds.
Presented at the 6th Meeting of the Asian Pacific Society of Applied Phycology, Manila, Philippines. |
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Keywords: | Amino acids Chlorophyceae Cladophora vagabunda Glycine betaine Osmoticant Protein profile |
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