Immunological Cross-Reactivity among Corresponding Proteins of Photosystems I and II from Widely Divergent Photosynthetic Organisms

Immunological cross-reactivity among corresponding proteinsassociated with photosystems I and II in higher plants, greenalgae, red algae and cyanobacteria were examined with antiseraraised against the proteins from Synechococcus elongatus andspinach. (1) Generally, the cross-reactivity was very high betweenclosely related species but decreased with increasing phylogeneticdistances between organisms. Exceptionally, proteins from redalgae showed lower reactivities with the antisera against thecyanobacterial proteins than did corresponding proteins fromgreen algae and higher plants. (2) The extent of the cross-reactivitywas found to vary with the antisera used. Three antisera preparedagainst large chlorophyll-carrying proteins of photosystem Iand photosystem II reaction center complexes of Synechococcusreacted with the corresponding proteins of all the organismsexamined. By contrast, an antiserum raised against the extrinsic35 kDa protein of the cyanobacterium reacted with none of corresponding33 kDa proteins of other species. The antiserum against thespinach 33 kDa protein showed a wider range of cross-reactivity.Antisera raised against the Dl and D2 proteins from spinachwere highly reactive with corresponding proteins from otherphotosynthetic organisms, whereas an antiserum against a well-conservedsequence of the spinach D2 protein showed limited cross-reactivity.The results show that, although the extent of immunologicalcross-reactivity is determined mainly by the homology betweenproteins, caution is indicated in the application of immunologicalmethods to determinations of the distribution of various proteinsrelated to photosystems I and II in very different organisms. (Received December 8, 1989; Accepted March 12, 1990)