| 蜜环菌遗传测定的单孢分离和培养方法 |
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| 引用本文： | 赵俊 田淑敏. 蜜环菌遗传测定的单孢分离和培养方法[J]. 微生物学通报, 1999, 26(3): 207-209 |
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| 作者姓名： | 赵俊 田淑敏 |
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| 作者单位： | 国家林业局森林病虫害防治总站!沈阳110034 |
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| 基金项目： | 国家自然科学基金青年基金!39500001 |
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| 摘 要： | 从平板中直接分离担子菌萌发孢子的单孢分离方法，主要利用自制的毛细管和显徽玻璃针等简单工具操作。该法简便快速，抗污染，获得单孢率高。同时报道一种改进型的担子菌互交不育性测定用培养基MEJA，与国内外目前常用的SR和MEA培养基相比，增加一项判断标准，使互交不育性和互交可育性的反应更加清晰可靠。
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| 关 键 词： | 蜜环菌 单孢分离 培养基 |
A METHOD OF SINGLE SPORE ISOLATION AND CULTURE FOR ARMILLARIA GENETIC TEST |
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| Zhao Jun,Tian Shumin, Wang Yuling, Qin Guofu. A METHOD OF SINGLE SPORE ISOLATION AND CULTURE FOR ARMILLARIA GENETIC TEST[J]. Microbiology China, 1999, 26(3): 207-209 |
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| Authors: | Zhao Jun Tian Shumin Wang Yuling Qin Guofu |
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| Affiliation: | General Station of Forest Pest Control. National Forestry Bureau. Shenyang 110034 |
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| Abstract: | A method are described for isolation of single spore. Under the microscope, the germinated single spore of the Aumillaria and other Agaricales can be isolated directly by a handmade pipette and a glass microneedle. the method modified from Kari Korhonen's and operation are better than former. As same times, A improved medium MEJA for intersterility experiments of Armillaria are reposed, by added of fresh malt extract juice to the melt extract powder which were adopted widely, the medium make compatible and incompatible reaction clarify exactly. |
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| Keywords: | Armillaria Single-spore isolation Medium |
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