金属硫蛋白产生菌的诱变育种

采用亚硝基胍和紫外线诱变，自金属硫蛋白（ＭＴ）产生菌酿酒酵母（ｄｅｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）ＢＤ１０１－２５单倍体中获得遗传稳定的高Ｃｕ２＋、Ｃｄ２＋抗性突变株ＢＤ１０１－６９和ＢＤ１０１－３０。并对其重金属解毒、桔抗ｕ．ｖ．和６０Ｃｏ辐射效应、清除羟基自由基能力等生物学功能进行了研究。与出发菌株相比，上述生物学活性与酵母细胞对Ｃｉ２＋抗性、ＭＴ表达量表现出正相关性。两个突变株类ＭＴ表达量与生物学活性皆有所提高，为酿酒酵母ＭＴ的理论和应用研究打下了基础。

SELECTION OF MUTANTS FROM METALLOTHIONEIN PRODUCING STRAIN

Two mutants of stranins BD101-69,BD101-30 having high resistance to Cu2 and Cd2 were obtainedfrom monoploids of metallothionein prodocing strain Saccharomyces cerevisiae BD101-25 after mutation withNTG and u. v .Analysis and comparision of the biological fumction were performed between these strains . Itwas shown that there was a positive correlation between the heavy metal ion detoxification, protection against u. v. and ray irradiation damage, elimination of hydroxyl radicals and yeast MT content, the resistance ofyeast cells to Cu2 . Two mutants showed higher biological activity and Cu-MT content.