小型无绿藻的分离及形态特征

无绿藻（Ｐｒｏｔｏｔｈｅｃａ）是一种少见的条件致病菌^［１］，可引起人畜共患的无绿藻病。在其所致人类疾病中，主要有三种临床类型：皮肤损害、局限性感染、系统性无绿藻病^［２］。本文于１９９７年从一名女患者面部丘疹处的皮肤结节活检，分离培养出一株无绿藻菌，并进行了分离培养鉴定，利用光学显微镜、电子显微镜对其形态特征和超微结构进行观察，并通过直接组织压片和组织病理切片观察和生化试验特性，确定为一例由小型无绿藻（Ｐｒｏｔｏｔｈｅｃａｗｉｃｋｅｒｈａｍｉｉ

PROTOTHECOSIS AND EXPERIMENTAL RESEARCH ON PROTOTHECA WICKERHAMII

Prototheca is a rare opportunistic pathogenic fungus, which can cause animal and human disease known as protothecosis. There are three clinical types in human protothecosis: dermal lesion, meningitis andsystematic protothecosis. A strain of Prototheca was isolated from a biopsied nodule specimen on the face of awoman patient in 1997, which was isolated and cultured on the basis of experimental studies of its physiologicalcharacters, examined under optic and electronic microscopes for its morphological and ultra-structural characteristics.The ofganism was also examined biochemically through direct tissue tabletting and histopathologic section and thiscase of face infection was finally identified as protothecosis caused by Prototheca wickerhamii. The biologicalcharacters of prototheca and the relating human diseases were also discussed in many aspects.