| SINPV基因组酶切图谱及多角体基因的序列分析 |
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| 引用本文： | 魏永杰, 龙綮新, 陈尚武, 王珣章,. SINPV基因组酶切图谱及多角体基因的序列分析[J]. 微生物学通报, 1999, 26(2): 88-92 |
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| 作者姓名： | 魏永杰 龙綮新 陈尚武 王珣章 |
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| 作者单位： | 中山大学昆虫学研究所、生物防治国家重点实验室; |
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| 基金项目： | 国家自然科学基金!No．39730030 |
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| 摘 要： | 用限制性内切酶ＥｃｏＲＩ、ＸａｂＩ、ＸｈｏＩ、ＢａｍＨＩ、ＰｓｔＩ、ＳａｃＩ、ＨｉｄｎⅢ、ＳｍａＩ酶解斜纹夜蛾核多角体病毒广州株基因组ＤＮＡ，分别得到２６、２６、２４、２０、１３、１７、９、１条片段，并算得基因组平均大小为１３６．０ｋｂｐ。以ＡｃＮＰＶ多角体基因的部分读码框为探针，经Ｓｏｕｔｈｅｍ杂交将ＳＩＮＰＶ多角体基因定位于ＸｂａＩＯ片段上。将此片段克隆并序列分析，结果表明ＳＩ
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| 关 键 词： | 斜纹夜蛾核型多角体病毒 限制性内切酶图谱 多角体基因 |
RESTRICTION PATTERNS AND NUCLEOTIDE SEQUENCE OF THE POLYHEDRIN GENE OF SPODOPTERA LITURA NUCLEAR POLYHEDROSIS VIRUS |
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| Wei Yongjie, Long Qixin , Chen Shangwu, Wang Xunzhang. RESTRICTION PATTERNS AND NUCLEOTIDE SEQUENCE OF THE POLYHEDRIN GENE OF SPODOPTERA LITURA NUCLEAR POLYHEDROSIS VIRUS[J]. Microbiology China, 1999, 26(2): 88-92 |
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| Authors: | Wei Yongjie Long Qixin Chen Shangwu Wang Xunzhang |
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| Abstract: | Digestion of Spodoptera litura nuclear polyhedrosis virus (SINPV Guangzhou isolate) with restrictionendonucleases EcoRI.XbaI.Xhol.Bam HI Pst I SacI .Hind and SmaI resulted in 26.26.24.20. 13. 17.9 and 1fragments, respectively. The average size of the genome is about 136.OKbp. The polyhedrin gene of SINPV waslocated on the Xba I O fragments by southem hybridization methed using a DIG labeled AcMNPV polyhedringene as probe. With a coding sequence of 780 nucleotides, corresponding to a protein of 249 amino acid, thepolyhedrin gene of SINPV is the longest polyhedrin gene known to date together with the Spodoptera littoralisNPV polyhedrin gene. This gene codes for a putative protein with an Mr 29236 and the predicted amino acidsequence shows high homology with those of other NPVs. |
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| Keywords: | Spodoptera litura Nuclear Polyhedrosis Virus Restriction endonuclease patterns Polyhedrin gene The nucleotide sequence of polyhedrin gene of SINPV has been submitted to the GeneBank/EMBL Data libraries under the accession number AF 037262 |
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