酸奶中乳酸菌镜检涂片的特殊染色法

报道了酸奶中乳酸菌涂片的特殊染色法-甲苯胺蓝染色法。涂片固定后，用0．2％甲苯胺蓝染色液染色2min，然后，用1％乙酸溶液脱色数秒，即可使涂片中的菌体着奥蓝色，而背景呈现白色或无色，菌体形状十分清晰。即使涂片很厚且不均，也可清晰看到菌体。便于菌体细胞的计数，形态观察及摄影．

STUDIES ON THE NEW SPECIAL COLOURING METHOD FOR SMEAR OF LACTIC ACID BACTERIA IN YOGURT

This article established a new special colouring method for smear of lactic acid bacteria in yogurtToluidine Blue Colouring Method. This method can dye the cell of microbian as blue with toluidine blue solutio(0.2%) for 2 min. Meanwhile, the light cologned medium of yogurt of yogurt or other material can be decolouredwith acehc acid (0.l%) for 2 Sec. Under the microscope, the smear of yogurt shows the blue cell of hocrobianin white or transparent background, even if the smear is very thick, the cell is observed clearly.