牙髓紫卟啉菌内毒素对炎症性细胞因子的介导作用

牙髓紫叶琳菌ＡＴＣＣ３５４０６是近年来新发现的重要致病性专性厌氧菌，采用改良酚－氯仿－石油醚法提取牙髓紫卟啉菌ＡＴＣＣ３５４０６内毒素脂多糖，通过Ｋｒａｍｅｒ测定法、软琼脂细胞培养法以及胸腺细胞增殖法测定脂多糖的细胞生物学活性。结果显示：纯化脂多糖可不同程度地诱导小鼠模型生成肿瘤坏死因子（ＴＮＦ）、集落刺激因子（ＣＳＦ）和白介素－１（ＩＬ－１），并在一定范围内呈剂量依赖型，提示牙髓紫卟啉菌内毒素在动物模型和细胞模型中具有显著的细胞生物学和免疫学活性．

PRODUCTION OF CYTOKINS INDUCED BY ENDOTOXIN PREPARED FROM PORPHYROMONAS ENDODONTALIS ATTCC 35406

cyto-immunological activities of endotoxin prepared from Porphyromonas ATCC 35406 were examed inproduction of interlukin-1(IL-1), tumor necrosis factor(TNF), colony stimulating factor(CSF) and Shwartzmanpreparatory Dose50 (SPD50). The results demonstrated that IL-1 can be produced at dose 0.1ng of endotoxin. Andlevel of IL-1 correlated with the dose od endotoxin; The endotoxin can also induce production of sera TNF atdose of 1.0ug in mice, TNF can be found in sera of mice after endotoxin injection one hour later, anddisappeared within six hours; the resultS still revealed this endotoxin have a inducing activity on macrophages of mouse in production of CSF at the dose 0.1ug and have a character of dose dependence; the SPD50 ofPorphyromonas endotoxin was 64ug in local Shwartzman reaction. Those results demonstrated the inducingactivities of Porphyromonas endotoxin on cytokins may be inportant for its pathogenisis in clinical infection.