Association with HeLa cells of LPS mutants ofSalmonella typhimurium andSalmonella minnesota in relation to their physicochemical surface properties |
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Authors: | Erik Kihlström Karl-Eric Magnusson |
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Institution: | 1. Department of Medical Microbiology, University of Link?ping, S-581 85, Link?ping, Sweden
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Abstract: | Different LPS mutants ofSalmonella typhimurium andSalmonella minnesota have been investigated with respect to (1) their tendency to associate, with HeLa cell monolayers, and (2) their physicochemical
surface properties. Aqueous biphasic partitioning, hydrophobic interaction chromatography, and ion exchange chromatography
have been used to characterize the bacterial cell surface properties with respect to charge and hydrophobicity. Liability
to hydrophobic interaction was defined either by the change of partition in a dextran-polyethylene-glycol (PEG) system by
the addition of PEG-palmitate (P-PEG), or by the elution pattern from Octyl-Sepharose. Accordingly, charge was assessed by
the effect of positively charged trimethylamino-PEG (TMA-PEG) on the partition, and by the elution from DEAE-Sephacel.
Bacterial being negatively charged and liable to hydrophobic interaction had the highest tendency to associate with HeLa cells.
In some cases the methods for surface analysis gave conflicting results on charge and/or liability to hydrophobic interaction
of the same LPS mutant. Possible reasons for these differences and the role of bacterial cell surface structures contributing
to physicochemical character are discussed. |
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Keywords: | Salmonella bacteria association with HeLa cells HeLa cells association with Salmonella bacteria aqueous two-phase partitioning of Salmonella bacteria hydrophobic interaction chromatography of Salmonella bacteria ion exchange chromatography of HeLa cells associated with Salmonella bacteria physicochemical surface properties of HeLa cells associated with Salmonella bacteria |
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