| 烟草黄酮醇合成酶基因的克隆及其序列分析 |
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| 引用本文： | 黄伟伟,杨曦,张常娥,方斌,马靓,常俊丽,杨广笑,何光源. 烟草黄酮醇合成酶基因的克隆及其序列分析[J]. 植物生理学通讯, 2006, 42(6): 1059-1062 |
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| 作者姓名： | 黄伟伟 杨曦 张常娥 方斌 马靓 常俊丽 杨广笑 何光源 |
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| 作者单位： | 华中科技大学中英HUST-RRes基因工程和基因组学联合实验室,武汉,430074 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金 |
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| 摘 要： | 根据已知的黄酮醇合成酶cDNA保守序列设计引物，用RT-PCR技术从烟草叶片中扩增获得黄酮醇合成酶cDNA片段，再用RACE方法得到其两端序列。根据获得的序列，设计引物分离得到完整的1188bp的黄酮醇合成酶基因，其开放阅读框编码346个氨基酸。序列分析显示，烟草黄酮醇合成酶与高杯花、矮牵牛和马铃薯的同源性分别为87％、86％和84％，与其它物种中的同源性也在80％左右，表明不同物种中黄酮醇合成酶基因具有高度同源性。此外，在氨基酸水平上，该酶与其它依赖于2-酮戊二酸的双加氧酶及其相关酶也具有同源性。
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| 关 键 词： | 烟草 黄酮醇合成酶基因 序列分析 |
| 收稿时间： | 2006-07-11 |
| 修稿时间： | 2006-11-16 |
Cloning and Sequence Analysis of Flavonol Synthase Gene from Tobacco (Nicotiana tabacun L.) |
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| HUANG Wei-Wei,YANG Xi,ZHANG Chang-E,FANG Bin,MA Liang,CHANG Jun-Li,YANG Guang-Xiao,HE Guang-Yuan. Cloning and Sequence Analysis of Flavonol Synthase Gene from Tobacco (Nicotiana tabacun L.)[J]. Plant Physiology Communications, 2006, 42(6): 1059-1062 |
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| Authors: | HUANG Wei-Wei YANG Xi ZHANG Chang-E FANG Bin MA Liang CHANG Jun-Li YANG Guang-Xiao HE Guang-Yuan |
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| Affiliation: | China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China |
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| Abstract: | According to the conserved cDNA sequence of flavonol synthase(FLS)gene,a pair of primers was designed,and FLS was cloned from tobacco(Nicotiana tabacun L.)leaves using RT-PCR and RACE.The full length cDNA sequence was 1 188 bp and it included a open reading frame(ORF)which encoded 346 residues.Sequence analysis showed that homologies of FLS cDNA from tobacco with Nierembergia caernlea,Petunia hybrida and Solanum tuberosum were 87%,86% and 84%,and which among the others were about 80%.The results indicated that FLS was highly stable in evolution of plants.On the other hand,it also had similarity with other 2-oxoglutarate-dependent dioxygenases on amino acids level. |
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| Keywords: | RACE |
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