Reisolation of the carbon monoxide utilizing hydrogen bacterium Pseudomonas carboxydovorans (Kistner) comb. nov. |
| |
Authors: | Ortwin Meyer Hans Günter Schlegel |
| |
Institution: | (1) Institut für Mikrobiologie der Universität Göttingen, Grisebachstr. 8, D-3400 Göttingen, Federal Republic of Germany |
| |
Abstract: | From enrichment cultures four carbon monoxide utilizing bacteria were isolated; strain OM5 isolated from waste water was studied in detail. The cells are Gram-negative, slightly curved rods, motile by a single subpolarly inserted flagellum. The colonies are smooth, translucent and not slimy.The cells are able to grow autotrophically in mineral medium under an atmosphere of 40% CO, 5% O2 and 55% N2 at a doubling time of 20h (30°C) or of 85% H2, 5% O2 and 10% CO2 at a doubling time of 7h. Heterotrophic growth occurrd on organic acids such as acetate (t
d
=8h), pyruvate (t
d
=8h), lactate, crotonate, malate, succinate (t
d
=8h), formate (t
d
=35h) and glyoxylate as substrates.The enzyme system for carbon monoxide utilization is formed only during growth on CO; hydrogenase is present in cells grown on CO or on H2+CO2 as well as grown on pyruvate. The rate of oxygen reduction by intact CO-grown cells is 3.7-fold higher in the presence of hydrogen than in the presence of carbon monoxide. During growth the stoichiometry of gas uptake was 6.1 CO+2.8 O2+H2O CH2O +5.1 CO2. For the new isolate the name Pseudomonas carboxydovorans (Kistner) comb. nov. has been proposed.Part of this work was presented at the Second International Symposium on Microbial Growth on C1-Compounds in Puschino, USSR, September 12th–16th, 1977. |
| |
Keywords: | Autotrophic growth Hydrogen Carbon monoxide Gram-negative hydrogen bacteria Pseudomonas carboxydovorans Facultative autotrophs CO oxidation |
本文献已被 SpringerLink 等数据库收录! |
|