Genetic Diversity of the Giant Tiger Shrimp (Penaeus monodon) in Thailand Revealed by PCR-SSCP of Polymorphic EST-Derived Markers |
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| Authors: | Sirawut Klinbunga Rachanimuk Preechaphol Supaporn Thumrungtanakit Rungnapa Leelatanawit Takashi Aoki Padermsak Jarayabhand Piamsak Menasveta |
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| Institution: | (1) Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand;(2) National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand;(3) Program of Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand;(4) Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 100-8477, Japan;(5) Aquatic Resources Research Institute, Chulalongkorn University, Bangkok, 10330, Thailand;(6) Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand |
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| Abstract: | A total of 90 ESTs from normal and 157 from subtractive ovarian cDNA libraries of the giant tiger shrimp (Penaeus monodon) were sequenced. SSCP analysis of disulfide isomerase (DSl), zinc finger protein (ZFP), PMO920, and PMT1700 was carried out for population genetic studies of P. monodon in Thai waters. The number of codominant alleles per locus for overall samples was 6 for PMO920, 5 for PMT1700, and 12 for ZFP, and there were 19 dominant alleles for DSI. The observed heterozygosity of each geographic sample was 0.3043–0.5128 for PMO920, 0.3462–0.4643 for PMT1700, and 0.5000–0.8108 for ZFP. Linkage disequilibrium analysis indicated that genotypes of these loci segregate randomly (P > 0.05). Low genetic distance was found between pairs of geographic samples (0.0077–0.0178). The neighbor-joining tree constructed from the average genetic distance of overall loci allocated the Andaman samples (Satun, Trang, and Phangnga) into one cluster, and Chumphon and Trat into other clusters. Geographic differentiation between Satun-Trat and Satun-Phangnga was found only at the ZFP locus (P < 0.05), suggesting low degrees of genetic subdivision of Thai P. monodon. |
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| Keywords: | genetic diversity SSCP EST Penaeus monodon shrimp |
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