Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification |
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| Authors: | Ro Hyeon-Su Jung Sun Ok Kho Byung Hoon Hong Hyung Pyo Lee Jae Sung Shin Yong-Beom Kim Min Gon Chung Bong Hyun |
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| Institution: | BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Korea. |
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| Abstract: | A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins. |
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