Trypan Blue as a Stain for Fungi

Trypan blue was tested for use in the study of fungi. It was found that it stained the cell wall of all fungi investigated. Two staining solutions were very satisfactory: (1) A quick-staining solution of 0.1-0.5% dye in 45% acetic acid; (2) A slow-staining solution of 0.1-0.5% dye in lactophenol. In both instances, heating the preparations quickened the action.