膳食甘油二酯对SD大鼠食欲及下丘脑神经肽Y基因表达的影响

研究膳食甘油二酯对SD大鼠食欲和下丘脑神经肽Y基因表达的影响。方法:将大鼠随机分成三组:5%(wt)甘油三酯组(对照组),20%(wt)甘油三酯组(TG组)和20%(wt)1,3—甘油二酯组(DG组)。大鼠喂养10W,每天记录进食量,每周称体重,于第10周末处死大鼠,称体重和内脏脂肪,计算脂体比,测定血糖、血脂和各种激素水平,RT-PCR的方法检测大鼠下丘脑神经肽Y mRNA表达水平。结果:高甘油二酯组与高甘油三酯组比较,改善体重的增加及内脏脂肪的积累,降低大鼠血糖、血甘油三酯(TG)、胰岛素水平,同时降低大鼠下丘脑神经肽Y mRNA的表达水平。结论:甘油二酯降低脂肪积累和改善血脂水平的可能机制是改变下丘脑有关控制食欲的基因表达,进而影响了脂肪代谢的平衡。

Effects of Dietary Diacylglycerol on Appetite and gene Expression of NPY in Rats

To investigate the effects of dietary diacylglycerol(DG)on lipid accumulation,appetite and the expression of hypothalamic gene related to energy homeostasis.Methods:Male Sprague-Dawley rats were randomly divided into 3 groups:50/0(wt)triacylglycerol(TG,control group),20%(wt)TG group and 20%(wt)DG group.All the rats were fed for 10 weeks.At the end of experiment,the rats were sacrificed,the ratio of lipids to body weight were calculated,and blood glucose,serum lipids,serum insulin were measured.RT-PCR was used for measuring the expression of neuropeptide Y(NPY)mRNA in the hypothalamus.Results:DG diets resulted in significant restraint of body weight gain,visceral fat,ratio of abdominal fat to body weight,and decreased serum triglyderide,glucose and insulin levels compared to High TG diets.Conclusion:These results suggest that diacylglycerol improved fat metabolism and reduced abdominal fats accumulation,which may be associated with improved appetite control and energy balance.