Sugar-binding activity of pea (Pisum sativum) lectin is essential for heterologous infection of transgenic white clover hairy roots by Rhizobium leguminosarum biovar viciae |
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Authors: | Ron R. van Eijsden Clara L. Díaz B. Sylvia de Pater Jan W. Kijne |
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Affiliation: | (1) Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, Netherlands;(2) Center For Phytotechnology RUL-TNO, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, Netherlands;(3) Present address: Department of Molecular Cell Biology, Section of Molecular Cytology, BioCenter, The University of Amsterdam, Plantage Muidergracht 14, 1018 TV Amsterdam, Netherlands |
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Abstract: | Legume lectin stimulates infection of roots in the symbiosis between leguminous plants and bacteria of the genus Rhizobium. Introduction of the Pisum sativum lectin gene (psl) into white clover hairy roots enables heterologous infection and nodulation by the pea symbiont R. leguminosarum biovar viciae (R.l. viciae). Legume lectins contain a specific sugar-binding site. Here, we show that inoculation of white clover hairy roots co-transformed with a psl mutant encoding a non-sugar-binding lectin (PSL N125D) with R.l. viciae yielded only background pseudo-nodule formation, in contrast to the situation after transformation with wild type psl or with a psl mutant encoding sugar-binding PSL (PSL A126V). For every construct tested, nodulation by the homologous symbiont R.l. trifolii was normal. These results strongly suggest that (1) sugar-binding activity of PSL is necessary for infection of white clover hairy roots by R.l. viciae, and (2) the rhizobial ligand of host lectin is a sugar residue rather than a lipid. |
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Keywords: | heterologous infection host specificity nodulation pea lectin sugar-binding |
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