| 一种快速筛选产琥珀酸厌氧菌的方法 |
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| 引用本文： | 陈设,詹晓北,陈蕴,郑志永,张丽敏.一种快速筛选产琥珀酸厌氧菌的方法[J].微生物学通报,2007,34(2):0315-0318. |
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| 作者姓名： | 陈设 詹晓北 陈蕴 郑志永 张丽敏 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,无锡,214036 |
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| 基金项目： | 无锡市学术技术带头人培养工程资助项目 |
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| 摘 要： | 为筛选得到高产琥珀酸的厌氧菌株,建立了一种快速半定量分析发酵液中琥珀酸的方法。首先通过高效液相色谱法(HPLC)分析出产琥珀酸厌氧菌发酵液中主要含有琥珀酸,乳酸和乙酸,然后将发酵液经过纸层析展开后,发现琥珀酸可以和副产物乳酸,乙酸完全分开,最后用半定量的方法求出琥珀酸的含量。结果表明纸层析法简单经济,可以作为产琥珀酸厌氧菌的初筛方法。
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| 关 键 词： | 琥珀酸 厌氧微生物 纸层析 高效液相色谱 |
| 文章编号： | 0253-2654（2007）02-0315-04 |
| 修稿时间： | 2006-03-31 |
A rapid Screening Procedure for Identification of Succinate Producing Anaerobic Strains |
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| CHEN She,ZHAN Xiao-Bei,CHEN Yun,ZHENG Zhi-Yong and ZHANG Li-Min.A rapid Screening Procedure for Identification of Succinate Producing Anaerobic Strains[J].Microbiology,2007,34(2):0315-0318. |
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| Authors: | CHEN She ZHAN Xiao-Bei CHEN Yun ZHENG Zhi-Yong and ZHANG Li-Min |
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| Institution: | The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036 |
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| Abstract: | In order to obtain an efficient screening procedure for identification of succinate producing anaerobic strains,a semi-quantitative paper chromatography method was developed. Lactic acid and acetic acid were identified as the main byproducts in the process of succinate production by the high performance liquid chromatography (HPLC).Succinic acid was completely separated from the byproducts of lactic acid and acetic acid in the same broth developed by paper chromatography.The content of succinic acid was calculated by a semi-quantitative method.The results showed that paper chromatography was a simple and cost effective method that could be utilized to screen anaerobic strains producing succinic acid. |
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| Keywords: | Succinic acid Anaerobic organisms Paper chromatography HPLC |
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