DNA synthesis in permeabilized karyoplasts from cytochalasin B-enucleated mouse L cells

DNA synthesis was examined in karyoplasts permeabilized by hypotonic buffer or lysolecithin. DNA synthesis was not affected by the omission of a single deoxyribonucleated triphosphate but was greatly reduced in the absence of three of the deoxyribonucleotide triphosphates. The rate of DNA synthesis was linear for 40 min and continued for up to 2–3 h. The DNA synthesized in the permeabilized karyoplasts was a continuation of semi-conservative replication occurring in the intact cell.