核桃楸叶片中总黄酮的最佳提取工艺研究

利用超声波法对核桃楸叶片中总黄酮的提取工艺进行了研究，在单因素试验的基础上，采用正交试验法，确立最佳提取条件，考察乙醇浓度、提取温度、液料比和提取时间对核桃楸叶片总黄酮提取率的影响。结果表明：超声波法辅助提取核桃楸叶片总黄酮的最适工艺参数是浸提剂乙醇浓度为70%，提取温度为50℃，液料比为20：1，提取时间为20 min，且总黄酮提取率可达4.458%。

Optimum Extraction Process of Total Flavonoids in Juglans mandshurica Leaves

The method of extraction total flavonoids from the leaves of Juglans mandshurica was optimized with the assistant of ultrasonic energy. The optimum extraction conditions of total flavonoids in J. mandshurica leaves were determined with orthogonal test. Four factors below were tested according to the extraction yield: ethanol concentration, extraction temperature, the ratio of material to solvent and extraction time. The optimum conditions of extracting were obtained as follows: 70% ethanol as extraction solvent at 50℃ for 20 min, and the ratio of solvent to material was 20:1 and the average extraction yield of total flavonoids from the leaves of J. mandshurica could be reached 4.458% under the optimum process.