Combined effects of ethanol and manganese on cultured neurons and glia |
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Authors: | M. Ledig G. Tholey L. Megias-Megias P. Kopp F. Wedler |
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Affiliation: | (1) Centre de Neurochimie du CNRS, 5, rue Blaise Pascal, 67084 Strasbourg Cedex, France;(2) University of Granada, Spain;(3) The Pennsylvania State University, University Park, Pennsylvania, U.S.A. |
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Abstract: | Manganese is essential for normal development and activity of the nervous tissue. Mn2+ ions are involved in protein synthesis and may prevent free radical damage. Since it is now established that alcohol degradation may produce free radicals, we studied the effect of Mn2+ on ethanol induced alterations using cultured nerve cells as an experimental model of the central nervous system. Neurons and glial cells were cultured from rat brain cortex; a tumoral rat glial cell line (C6) was also examined. We measured enzymatic markers of nerve cell maturation (enolase, glutamine synthetase) and superoxide dismutase, a scavenger of free radicals; all these enzymes being activated by Mn2+ ions. Only for the glial cell types an alcohol antagonizing effect was found when Mn2+ was combined with ethanol. Neurons were not sensitive to that Mn2+ effect. |
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Keywords: | Ethanol manganese cell cultures neurons glial cells enzymes |
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