Rapid identification of vinca alkaloids by direct-injection electrospray ionisation tandem mass spectrometry and confirmation by high-performance liquid chromatography-mass spectrometry |
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Authors: | Zhou Hui Tai Yuanpo Sun Cuirong Pan Yuanjiang |
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Institution: | Department of Chemistry, Zhejiang University, Hangzhou, People's Republic of China. |
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Abstract: | A simple and rapid method for the identification of Vinca alkaloids from a crude extract of Catharanthus roseus G. Don (Apocynaceae) by direct-injection electrospray ionisation (ESI) and tandem mass spectrometry (MS/MS) has been developed. The alkaloids vindoline, vindolidine, vincristine and vinblastine were evaluated in a commercial extract of C. roseus using this method. Catharanthine and its isomers 19S-vindolinine and vindolinine were detected in the commercial product by direct injection ESI/MS/MS and confirmed by preparation and by HPLC-ESI/MS. For the characterisation of different fragment fingerprints, ESI/MS/MS is a sensitive, rapid and convenient technique by which to identify some constituents in complex and mixed plant extracts. |
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Keywords: | ESI/MS MS/MS HPLC‐MS identification direct‐injection vinca alkaloids Catharanthus roseus |
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