Cloning of Epstein-Barr virus (EBV) DNA fragments in pBR32 and Charon3A |
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| Authors: | S Yano H E Faber Y S Lee M Nonoyama |
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| Institution: | 1. Laboratory of Molecular Virology, Life Science Biomedical Research Institute Inc., 2900 72nd Street North, St. Petersburg, FL 33710 U.S.A.;7. Molecular Genetics Laboratory, Papanicolaou Cancer Research Institute, 1155 Northwest 14th Street, P.O. Box, 01-6188, Miami, FL 33101 U.S.A. |
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| Abstract: | Restriction fragments of Epstein-Barr virus (EBV; B95-8) DNA were cloned in the Tc gene of pBR322 (HindIII-F, -G, -I, -J, -K, -L, and -M) and in Charon3A (EcoRI-GI and -G2). Altogether these cloned fragments covered 39% of the entire viral genome. The cloned EcoRI-G2 fragment of EBV (B95-8) DNA was shown to contain, in addition to HindIII-J, two more HindIII-fragments : HindIII-M, which had not been located on the linkage map of the viral genome (Given and Kieff, 1978) and HindIII-N, which had been unrecognized up to now. The utility of this cloning method is discussed in regard to the detailed mapping of a viral genome and large-scale production of the viral DNA. |
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| Keywords: | Recombinant DNA restriction endonucleases mapping large-scale production plasmid and phage λ vectors bp base pairs EBV Epstein-Barr virus Md megadaltons |
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