An improved method to isolate lichen algae by gel filtration |
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Authors: | Mará José Pérez Carlos Vicente María Estrella Legaz |
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Affiliation: | (1) Department of Plant Physiology, The Lichen Team, Faculty of Biology, Complutense University, E-28040 Madrid, Spain |
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Abstract: | Photobiont cells of the lichen Evernia prunastri have completely been separated from their fungal partner by filtration through a bed of Sepharose 2B. Both mannitol and ribitol have been quantified by gas-liquid chromatography in the different steps of the isolation procedure. Absence of mannitol, which is exclusively produced by the mycobiont, has been used as the best probe to monitor isolation. |
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