Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance. |
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| Authors: | J A Vara, A Portela, J Ortí n, A Jim nez |
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| Affiliation: | J A Vara, A Portela, J Ortín, and A Jiménez |
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| Abstract: | The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic. |
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