Hepatitis B virus transcripts in a human hepatoma cell line, Hep 3B |
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| Authors: | T S Su L H Lin C K Chou C M Chang L P Ting C P Hu S H Han |
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| Affiliation: | 1. Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China;2. Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China;3. School of Pharmaceutical Science, Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | Hep 3B, a human hepatoma cell line was examined for its RNA hybridizable to the hepatitis B virus sequence. Using probes that covered different regions of the hepatitis B virus genome, five species of RNA were observed of sizes 4.0, 3.3, 2.9, 2.6 and 2.2 kilobases. The RNAs covered surface antigen gene, pre-S and X regions. None of them had a core antigen sequence. RNA with a 4.0 kilobase size was the most abundant. Using S1 nuclease analysis, its 5' end of hepatitis B virus sequence was mapped at pre-S region and its 3' end of viral sequence was mapped at DR region. |
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