Strain typing of ectomycorrhizal basidiomycetes from subalpine Tyrolean forest areas by random amplified polymorphic DNA analysis |
| |
Authors: | S. B. Haudek F. Gruber N. Kreuzinger F. Göbl C. P. Kubicek |
| |
Affiliation: | (1) Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9/1725, A-1060 Wien, Austria Tel.: (43–1) 58 801–47 07; Fax: (43–1) 581–6266; e-mail: ckubicek@fbch.tuwien.ac.at, AT |
| |
Abstract: | The application of random amplified polymorphic DNA (RAPD) analysis for the identifcation of ectomycorrhizal symbionts of spruce (Picea abies) belonging to the genera Boletus, Amanita and Lactarius at and below the species level was investigated. Using both fingerprinting [M13, (GTG)5, (GACA)4] as well as random oligonucleotide primers (V1 and V5), a high degree of variability of amplified DNA fragments (band-sharing index 65–80%) was detected between different strains of the same species, hence enabling the identification of individual strains within the same species. The band-sharing index between different species of the same genus (Boletus, Russula and Amanita) was in the range of 20–30%, and similar values were obtained when strains from different taxa were compared. Thus RAPD is too sensitive at this level of relatonship and cannot be used to align an unknown symbiont to a given taxon. We therefore conclude that RAPD is a promising tool for the identification of individual strains, and could thus be used to distinguish indigenous and introduced mycorrhizal strains from the same species in natural ecosystems. Accepted: 29 August 1995 |
| |
Keywords: | Key words Ectomycorrhiza Boletus Amanita Lactarius Russula Picea abies RAPD Intra- and infraspecific variability |
本文献已被 SpringerLink 等数据库收录! |
|