Rapid removal of unincorporated label and proteins from DNA sequencing reactions |
| |
Authors: | Tadeusz Kaczorowski Marian Sektas |
| |
Institution: | (1) McArdle Laboratory for Cancer Research, University of Wisconsin, 1400 University Avenue, 53706 Madison, WI |
| |
Abstract: | This article presents a simple and rapid method for removal of unincorporated label and proteins from DNA sequencing reactions
by using Wizard purification resin. This method can be successfully applied for preparation of end-labeled oligonucleotides
free of unincorporated label, which is important in experiments (including DNA sequencing) when the level of background should
be as low as possible. Also, this method is effective in removal of proteins from DNA sequencing reactions. |
| |
Keywords: | Radiolabeling Sequenase 2 0 SPEL-6 Wizard resin |
本文献已被 SpringerLink 等数据库收录! |