Unit gravity sedimentation separation of cells comprising the caput epididymidis of the rat |
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| Authors: | G J Killian Jeanne Snyder R P Amann |
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| Institution: | (1) Dairy Breeding Research Center, The Pennsylvania State University, Pennsylvania, USA;(2) Department of Biological Sciences, Kent State University, 44242 Kent, Ohio, USA;(3) Department of Biochemistry, Southwestern Medical School, Dallas, Texas, USA |
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| Abstract: | Summary Cells from the rat caput epididymidis were separated by unit gravity sedimentation. Purest 12-ml fractions contained 88–95% basal cells or 64–76% principal cells. Ultrastructure of separated cells was similar to that of cells in intact tissue. Viability of separated cells was excellent as determined by dye exclusion tests and cellular ATP content. By combining fractions pools containing 4.0±0.9 × 106 cells (86±8% basal cells) and 1.4±0.4 × 106 cells (56±7% principal cells) were obtained. Thus, studies on the function of basal and principal cells from the rat caput epididymidis should be possible.This research was supported by NIH Grant HD-07244 and was authorized for publication as Paper No. 5231 in the Journal Series of the Pennsylvania Agricultural Experiment Station. We thank Mrs. J. Eastman for expert technical assistance |
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| Keywords: | Epididymis rat Epithelium isolation Unit gravity sedimentation |
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