Chicken myeloid stem cells infected by retroviruses carrying the v-fps oncogene do not require exogenous growth factors to differentiate in vitro |
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| Authors: | J F Carmier J Samarut |
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| Institution: | 1. Department of Bioengineering and Applied Physics, Stanford University, Stanford, CA 94305, USA;2. Department of Developmental Biology, Stanford University School of Medicine, CA 94305, USA;3. Chan Zuckerberg Biohub, San Francisco, CA 94158, USA;4. Institute of Cellular Therapeutics, Allegheny Health Network, 320 East North Avenue, Pittsburgh, PA 15212, USA;5. Department of Information Technology, Uppsala University, Sweden and SciLifeLab, Uppsala, Sweden SE-751 05;1. Laboratoire d’Anthropobiologie Moléculaire et d’Imagerie de Synthèse, CNRS UMR 5288, Université de Toulouse, Université Paul Sabatier, 31000 Toulouse, France;2. Lundbeck Foundation GeoGenetics Center, University of Copenhagen, 1350K Copenhagen, Denmark;3. Department of Biotechnology, Abdul Wali Khan University, Mardan, Pakistan;4. Evolutionary Ecology Group, Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK;5. Institute for Immunity, Transplantation and Infection, Stanford University, Stanford, CA 94305, USA;6. Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;7. Seminari d’Estudis i Recerques Prehistoriques, HAR2017-87695-P, Universitat de Barcelona, Barcelona, Spain;8. Anthropology Department, Hartwick College 1, Oneonta, NY 13820, USA;9. Shejire DNA project, 050046 Almaty, Kazakhstan;10. McDonald Institute for Archaeological Research, Department of Archaeology, University of Cambridge, Cambridge CB2 3ER, UK;11. National Museum of Mongolia, Ulaanbaatar 210646, Mongolia;12. Deutsches Archäologisches Institut (DAI), 14195 Berlin, Germany;13. Laboratorio de Paleontologia y Paleobiologia, Instituto Andaluz del Patrimonio Historico, Sevilla, Spain;14. Departamento de Prehistoria y Arqueología, Universidad Autónoma de Madrid, Madrid, Spain;15. Department of Paleolithic, National Museum of Iran, 1136918111, Tehran, Iran;16. Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biosciences, University of Oslo, Postbox 1066, Blindern, 0316 Oslo, Norway;17. Ecology Group, Department of Biology, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar 14201, Mongolia;18. Institute of Animal Breeding and Genetics, Department of Biomedical Sciences, Veterinary University of Vienna, 1210 Vienna, Austria;19. Palaeogenetics Group, Institute of Organismic and Molecular Evolution (iOME), Johannes Gutenberg-University Mainz, 55099 Mainz, Germany;20. Osteological material research laboratory, Klaipėda university, Klaipėda 92294, Lithuania;21. Department of Osteology, National Museum of Iran, 1136918111, Tehran, Iran;22. Department of Archaeology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran;23. Centro de Biología Molecular Severo Ochoa (CSIC-UAM), E-28049, Madrid, Spain;24. Integrative prähistorische und naturwissenschaftliche Archäologie (IPNA), 4055 Basel, Switzerland;25. Uniarq, Centro de Arqueologia da Universidade de Lisboa, Faculdade de Letras da Universidade de Lisboa, 1600-214 Lisboa, Portugal;26. CE3C-Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal;27. Institute for Pre- and Protohistoric Archaeology and Archaeology of the Roman Provinces, Ludwig-Maximilians-University Munich, 80539 München, Germany;28. ArchaeoBioCenter, Ludwig-Maximilians-University Munich, 80539 München, Germany;29. Institute of Palaeoanatomy, Domestication Research and History of Veterinary Medicine, Ludwig-Maximilians-University Munich, 80539 München, Germany;30. Explico Foundation, 6900 Florø, Norway;31. Department of Archaeology, Ulaanbaatar State University, Ulaanbaatar 51, Mongolia;32. Archaezoology section, Bioarchaeology Laboratory of the Central Laboratory, University of Tehran, Tehran CP1417634934, Iran;33. Departamento de Historia, Facultad de Filosofía y Letras, Universidad de León, León, Spain;34. Departamento de Sistemas Físicos, Químicos y Naturales, Universidad Pablo de Olavide, 41013 Sevilla, Spain;35. Operational Direction, Earth and History of Life, Royal Belgian Institute of Natural Sciences, 1000, Brussels, Belgium;36. Faculty of Agricultural and Environmental Sciences, The Agricultural University of Iceland, Keldnaholti - Árleyni 22, 112 Reykjavík, Iceland;37. University of Potsdam, Faculty of Mathematics and Natural Sciences, Institute for Biochemistry and Biology, 14476 Potsdam, Germany;38. Institute for the History of Material Culture, Russian Academy of Sciences, St. Petersburg 191186, Russia;39. Archaeology Institute of Samarkand, Uzbekistan;40. Institute of Plant and Animal Ecology, Urals Branch of the Russian Academy of Sciences, Ekaterinburg 620144, Russia;41. Department of Historical Studies, University of Gothenburg, Gothenburg, Sweden;42. Department of History, Kyrgyz-Turkish Manas University, Bishkek, Kyrgyzstan;43. Institut de Biologia Evolutiva, (CSIC-Universitat Pompeu Fabra), PRBB, Barcelona, Catalonia 08003, Spain;44. Samara State University of Social Science and Education, Samara, Russia;45. Department of Archaeology, Faculty of Humanities, University of Tehran, Iran;46. Conservation and Evolutionary Genetics Group, Estación Biológica de Doñana (EBD-CSIC), 41092 Sevilla, Spain;47. Laboratoire Archéorient, UMR 5133, Maison de l’Orient et de la Méditerranée, 69365 Lyon Cedex 7, France;48. Departamento de Prehistoria y Arqueología, Universidad Autónoma de Madrid, Madrid, Spain;49. Laboratory for Archaeological Research, Faculty of History and Law, Kostanay State University, Kostanay, Kazakhstan;50. Archaeological Research Collection, Tallinn University, 10130 Tallinn, Estonia;51. Department of Evolutionary Genetics, Leibniz Institute for Zoo and Wildlife Research, 10315 Berlin, Germany;52. Faculty of Life Sciences, Albrecht Daniel Thaer-Institute, Humboldt University Berlin, 10115 Berlin, Germany;53. Museu Nacional de História Natural e da Ciência, Universidade de Lisboa, Lisboa, Portugal;54. Centro Interuniversitário de História das Ciências e da Tecnologia (CIUHCT), Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal;55. Instituto Universitário de Lisboa (ISCTE-IUL), CIES-IUL, Lisboa, Portugal;56. Catalan Institution of Research and Advanced Studies (ICREA), 08010 Barcelona, Spain;57. CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain;58. Institut Català de Paleontologia Miquel Crusafont, Universitat Autònoma de Barcelona, Edifici ICTA-ICP, c/ Columnes s/n, 08193, Cerdanyola del Vallès, Barcelona, Spain;59. S.Toraighyrov Pavlodar State University, Joint Research Center for Archeological Studies, 637000 Pavlodar, Kazakhstan;60. University of Oxford, Faculty of History, George Street, Oxford, OX1 2RL, UK;61. Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle, Archéozoologie, Archéobotanique, Sociétés, Pratiques et Environnements (UMR 7209), 75005 Paris, France;62. Laboratory of Archaeozoology, Department Biología, Universidad Autónoma de Madrid, Madrid, Spain;63. Archaeology of Social Dynamics Group (ADS), Institució Milà i Fontanals-Consejo Superior de Investigaciones Científicas (IMF-CSIC), 08001 Barcelona, Spain;64. Grup d''Investigació Prehistòrica, HAR2016-78277-R, Universitat de Lleida, 25003 Lleida, Spain;65. Osteoarchaeology Practice and Research Center and Department of Anatomy, Faculty of Veterinary Medicine, Istanbul University-Cerrahpaşa, 34320, Avcılar, Istanbul, Turkey;66. Ivane Javakhishvili Tbilisi State University, Tbilisi 0179, Georgia;67. Université de Bordeaux, CNRS, UMR 5199-PACEA, 33615 Pessac Cedex, France;68. Institute for Anthropological Research, Gajeva 32, 10000 Zagreb, Croatia;69. Vinkovci Municipal Museum, 32 100 Vinkovci, Croatia;70. Iranian Center for Archaeological Research (ICAR), Iranian Cultural Heritage, Handicrafts, and Tourism Organization (ICHHTO), 1136918111, Tehran, Iran;71. School of History, Classics and Archaeology, The University of Edinburgh, Edinburgh, EH8 9AG, UK;72. Central Natural Science Collections (ZNS), Martin Luther University Halle-Wittenberg, Domplatz 4, 06108 Halle (Saale), Germany;73. INRAP Guadeloupe, Centre de recherches archéologiques, UMR 7209 CNRS/MNHN, 97113 Gourbeyre, Guadeloupe;74. Department of Ecology and Evolutionary Biology and Howard Hughes Medical Institute, University of California, Santa Cruz, Santa Cruz, CA 95060, USA;75. Saeedi Institute for Advanced Studies, University of Kashan, Kashan 87317-51167, Iran;76. Department Earth System Science, University of California, Irvine, Irvine, CA 92697, USA;77. Department of Archaeology, University of Nottingham, Nottingham, NG7 2RD, UK;78. Department of Archaeology, University of Exeter, Exeter, EX4 4QE, UK;79. Kermanshah Regional Water Authority, Kermanshah 67145-1466, Iran;80. Max Planck Institute for the Science of Human History, 07745 Jena, Germany;81. Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, Croatia;82. Department of Anthropology and Archaeology, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar 14201, Mongolia;83. Saryarka Archaeological Institute of Buketov Karaganda State University, Karaganda 100074, Kazakhstan;84. Faculty of Humanities (Archaeology), University of Southampton, Avenue Campus, Highfield, Southampton SO17 1BF, UK;85. Scientific Research Institute of Archaeology and Steppe Civilizations, Al Farabi Kazakh National University, 050040 Almaty, Kazakhstan;86. deCODE Genetics, 101 Reykjavik, Iceland;87. GABI UMR1313, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France;1. Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA;2. Department of Pathology, University of Washington, Seattle, WA 98195, USA;3. Department of Computer Science, University of Washington, Seattle, WA 98195, USA;4. Illumina, San Diego, CA 92122, USA;5. Brotman Baty Institute for Precision Medicine, Seattle, WA 98195, USA;6. Howard Hughes Medical Institute, Seattle, WA 98195, USA;1. Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA;2. Department of Cell Biology, Harvard Medical School, Boston, MA, USA;3. Broad Institute of MIT and Harvard, Cambridge, MA, USA;4. Department of Pathology, Brigham and Women’s Hospital, Boston, MA, USA;5. Massachusetts General Hospital, Cambridge, MA, USA;6. Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA, USA;7. Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA;8. Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital, Boston, MA, USA;9. Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, USA;10. Howard Hughes Medical Institute and Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA;1. BGI-Shenzhen, Shenzhen 518103, China;2. Department of Biology, University of Copenhagen, Copenhagen 2200, Denmark;3. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;4. Shenzhen Key Laboratory of Single-Cell Omics, BGI-Shenzhen, Shenzhen 518120, China;5. Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China;6. Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA;7. Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;8. MGI, BGI-Shenzhen, Shenzhen 518083, China;9. BGI College & Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450000, China;10. Shenzhen Bay Laboratory, Shenzhen 518000, China;11. Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), ICREA and CIBERNED, Barcelona 08003, Spain;12. Spanish National Center on Cardiovascular Research (CNIC), Madrid 28029, Spain;13. Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge CB2 0XY, UK;14. Guangzhou Laboratory, Guangzhou 510320, China;15. State Key Laboratory of Molecular Development Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;16. Department of Pathology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China;17. China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China;18. Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen 518120, China;19. Shenzhen Key Laboratory of Neurogenomics, BGI-Shenzhen, Shenzhen 518103, China;20. BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China;21. James D. Watson Institute of Genome Sciences, Hangzhou 310058, China;22. Medical Research Council Human Immunology Unit, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7BN, UK;23. Department of Protein Science, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm 17121, Sweden;24. Department of Neuroscience, Karolinska Institute, Stockholm 17177, Sweden;25. Institute of Stem Cells and Regeneration, Chinese Academy of Sciences, Beijing 100101, China;1. Department of Immunology, Weizmann Institute of Science, Rehovot, Israel;2. Flow Cytometry Unit, Department of Biological Services, Weizmann Institute of Science, Rehovot, Israel;3. Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel;4. CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria;5. Department of Medicine I, Laboratory of Infection Biology, Medical University of Vienna, 1090 Vienna, Austria;6. Department of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria;7. Department of Neuroscience, Karolinska Institutet, Retzius väg 8, 17177 Stockholm, Sweden |
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| Abstract: | To determine the function of c-fps in chicken macrophages and granulocytic cells we have infected chicken bone marrow cells with retroviruses containing the v-fps oncogene. Normal chicken macrophage progenitors, M-CFCs, give rise to macrophage colonies in semisolid cultures when macrophage colony stimulating factor (M-CSF) is added into the culture medium. Upon infection with v-fps bearing retroviruses, we observed that M-CFCs were induced to develop macrophage colonies in vitro without exogenous M-CSF. This activation results from a direct effect of v-fps on the M-CFCs. No leukemic transformation was observed in the infected colonies. By comparing the effects of several retroviruses, we showed that the induction of M-CFC development is specific to v-fps containing viruses and mediated by the v-fps protein. These observations support the hypothesis that the c-fps gene is involved in the control of proliferation and/or differentiation of myeloid cells. |
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